Enzymology: Enzyme kinetics

 

 

Carbohydrate metabolism

liverThe liver has a crucial role in blood glucose homeostasis, because the liver can release glucose from glycogen stores or synthesize glucose from gluconeogenetic precursors. Therefore, the control of hepatic glucose production has been targeted as a strategy for treatment of diabetes.

 

For reduction of hepatic glucose production glycogenolysis and gluconeogenesis can be suppressed by inhibition of the respective key-enzymes. MetaPhysiol offers to analyze the potential of your samples on inhibition of glycogenolytic and gluconeogenetic enzymes.

 

 

 

 

For further details, please contact MetaPhysiol

 


 

 

Glycogenolysis and Gluconeogenesis

 

Glycogen phosphorylase is assayed as key enzyme of glycogenolysis, while PEPCK and FBPase represent key enzymes of gluconeogenesis and G6Pase liberates glucose from G6P.

 

No. enzyme source methode substrate product literatur
030 glycogen-phosphorylase
EC 2.4.1.1)

 rabbit muscle spectrophotometric fluorimetric glycogen, inorganic phosphate glucose-1-P 1
031 fructose 1,6-bisphosphatase (EC 3.1.3.11) liver spectrophotometric fluorimetric F-1,6-P2 fructose 6-P, inorganic phosphate 1
032 PEPCK (EC 4.1.1.32) liver spectrophotometric fluorimetric OXA, GTP/ITP pyruvate, GDP/IDP 1
033 glucose 6-phosphatase
(EC 3.1.3.9)		 liver spectrophotometric fluorimetric G-6-P glucose, inorganic phosphate 1

 

 

 

Literature references

 

  1. Passonneau JV & Lowry OH (eds.), Enzymatic Analysis, Humana Press, Totowa, NJ, 1993
  2. Kurukulasuriya R. et al. Curr Med Chem 10, 123, 2003